Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues. EPIXTRACT Nuclear Protein Isolation Kit II provides a simple and reliable method to isolate nucleic acid-free nuclear proteins. Extracts can be used for a variety of downstream applications including western blotting, protein-DNA binding assays, nuclear enzyme assays and others requiring nucleic acid-free nuclear proteins. Assay Time: 1 hour. Contents: Pre-extraction Buffer, Extraction Buffer, Extraction pre-cleaner, Extraction cleaner, Protease Inhibitor Cocktail, DTT