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Core component of nucleosome Nucleosomes wrap and compact DNA into chromatin limiting DNA accessibility to the cellular machineries which require DNA as a template Histones thereby play a central role in transcription regulation DNA repair DNA replication and chromosomal stability DNA accessibility is regulated via a complex set of post-translational modifications of histones also called histone code and nucleosome remodeling
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The ChromaFlash Chromatin Isolation & Shearing Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format, followed by a shearing step by sonication. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash One-Step ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21 3
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